According to customer requirements can be customized research and development, in the range of 500 mL manufacturing of scientific retrovirus vector. It provides the whole solution of CDMO service from antibody screening, vector construction, viral vector packaging to CAR-T cell preparation and CAR-T cell activity verification in vivo and in vitro. At the same time, the company can provide CAR specific retroviral vector products targeting a variety of tumor targets, such as BCMA, CD19, CD38, CD22, EGFR/EGFRV III, which can efficiently transduce human primary T cells. Among them, CD38 CAR is the whole human anti-CD38 scFv structure screened by our phage display scFv antibody library, with independent intellectual property rights.

Adeno associated virus (AAV) is a kind of single strand DNA defective virus with simple structure and no envelope. It has the ability to infect divided or non divided cells, can meet the packaging of larger genes, is the preferred tool for overexpressing genes, and has the characteristics of good safety, wide host cell range, low epidemic resistance, and long time to express foreign genes in vivo.

Non-viral vector is the use of non-viral vector material physical and chemical properties to mediate gene transfer. Shenzhen Cell Valley mainly provides a unique non-viral genetic engineering technology (PiggyBac transposon modification), which is the core technology for the development of CAR T therapy. PiggyBac efficiently delivers large amounts of genetic material to T cells and can produce CAR-T products with a high percentage of dry memory cells. It includes four subtypes: single gene, single promoter double gene (IRES), double promoter double gene and double promoter double gene (WPRE).

The lentivirus vector is a virus vector system modified on the basis of HIV-1, which can effectively integrate foreign genes or foreign shRNA into the host chromosome, thus achieving the effect of persistent expression of the target sequence. Lentivirus has become one of the commonly used vector forms to express foreign genes or foreign shRNA, and is getting more and more widely used. Cell Valley can provide lentivirus vector customization services for a variety of tumor targets, such as BCMA, CD19, CD38, CD22, EGFR/EGFRVIIII, etc.

Astrocytes are the most abundant cell type in the brain and play an important role in regulating levels of extracellular ions and neurotransmitters, regulating synaptic activity and maintaining glutamate homeostasis, as well as maintaining the integrity of the blood-brain barrier. After trauma or infection, astrocytes are rapidly activated to form reactive astrocytes characterized by hyperplasia, hypertrophy, and high expression of intermediate filament proteins (including gliofibrillary acidic protein, vimentin, and nestin). Studies have shown that reactive astrocytes have both neurotoxic and neurotrophic effects. Shenzhen Cell Valley can provide the whole process services from neural stem cell differentiation Astrocytes, Astrocytes purification, quality control to release, with high product purity, quality control, high safety, scientific research cooperation with scientific research institutes, medical institutions, enterprises, etc., for the pre-clinical scientific research of Astrocytes. Innovative breakthrough to provide solutions.

Mesenchymal stem cells (mesenchymal stem cells) are important members of the stem cell family. They originate from the mesoderm in the early stage of development and are pluripotent stem cells. They have attracted much attention due to their characteristics of self-replication, self-renewal, multi-differentiation potential, hematopoietic support and promotion of stem cell implantation. In addition, mesenchymal stem cells have low immunogenicity and unique immunomodulatory effects, which can evade immune recognition, inhibit immune response, and have directional homing and damage chemotaxis. Shenzhen Cell Valley can provide high purity, high vitality of cells for basic and clinical research.

CAR-NK cells are allogeneic effector cells that can be easily expanded and modified, which can enhance the universality and simplicity of treatment, and make up for the weakness of autoimmune cells in patients with advanced cancer. CAR-NK cells kill target cells through CAR-dependent and NK cell receptor-dependent mechanisms that have eliminated tumor cells positive for tumor-associated antigens or tumor cells expressing NK cell receptor ligands. Shenzhen Cell Valley will provide the whole process service from NK cell collection, transduction to amplification, and the efficiency of transduction of foreign genes can reach more than 50%, which can help customers accelerate the research process in the field of CAR-NK.

CAR-M cell therapy refers to the transfer of a specific CAR gene that has been edited into macrophages so that it has the ability to bind to the tumor cell surface by specific antigen identification and subsequently activate macrophage activity against the tumor cells. Genetically engineered CAR-M cells can not only target phagocytic tumor cells, but also change the microenvironment near the tumor by secreting pro-inflammatory cytokines, and present tumor antigens to T cells to activate the immune response of T cells to the tumor. The emergence of CAR-M opens up a new possibility for the treatment of solid tumors: to train human macrophages with specific CAR to improve phagocytosis activity and macrophage antigen presentation against tumors. Shenzhen Cell Valley will work with customers to explore the most suitable CAR structure, foreign genes and transduction conditions for macrophages to help CAR M research and development.

1.Service content (1) Preclinical research Cell Valley can provide an integrated preclinical cellu...

Service content： 1. Plasmid construction Plasmids are DNA molecules that stain in vitro and have a...